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Fig. 2 | BMC Research Notes

Fig. 2

From: Selection of a suitable reference gene for quantitative gene expression in mouse lymph nodes after vaccination

Fig. 2

Evaluation of reference gene expression. a Gene expression by RT-qPCR. Average Cq values from duplicate qPCR reactions of each aLN (n = 30) and iLN sample (n = 29) were used for data analysis. The box indicates 25–75% with median shown in the box and the whiskers represent the minimum to maximum range. b Scores determined by RefFinder including the comprehensive stability, geNorm, NormFinder, BestKeeper and comparative delta-Ct method are shown for 7 reference genes. c Scores determined by RefFinder including the comprehensive stability, geNorm, NormFinder, BestKeeper and comparative delta-Ct method are shown for 4 reference genes

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