Fig. 4
Effect of reference genes on the calculated expression of Irf4 and Zbtb20.The relative expression of Irf4 (a) and Zbtb20 (b) in aLN and iLN were normalized with Actb, Ywhaz, Gapdh, Tbp, Ubc, Hprt or Hmbs by RT-qPCR after 1 and 7 days of the 1st and 3rd vaccination (V1D1, V1D7, V3D1 and V3D7, respectively). Data are presented as mean + SEM. Comparison of ΔΔCq values by one-way ANOVA and Tukey’s multiple comparison test was performed. Experimental groups identified with different symbols were significantly different from each other (P < 0.05)