TY - JOUR AU - Archer, Benedict G. PY - 2017 DA - 2017/12/11 TI - Note on the PCR threshold standard curve JO - BMC Research Notes SP - 731 VL - 10 IS - 1 AB - The PCR threshold standard curve is based on an exponential model of the initial phase of a PCR run where template replication efficiency is constant cycle to cycle. As such it requires that a threshold is at a level of amplified template not higher than where replication efficiency falls from its initial value. A second requirement is that all amplification profiles, both calibration and test, have the same initial efficiency. However, whether these requirements are met may not be checked, and there seems an apparent awareness that thresholds can be set higher than where efficiency has dropped from the initial value without compromising result validity. The objective of this study is to reconcile using the method without satisfying the requirement that amplification is exponential at threshold level. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-017-3036-4 DO - 10.1186/s13104-017-3036-4 ID - Archer2017 ER -