TY - JOUR AU - Basler, Nikolas AU - Xenikoudakis, Georgios AU - Westbury, Michael V. AU - Song, Lingfeng AU - Sheng, Guilian AU - Barlow, Axel PY - 2017 DA - 2017/12/20 TI - Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone JO - BMC Research Notes SP - 754 VL - 10 IS - 1 AB - A key challenge in ancient DNA research is massive microbial DNA contamination from the deposition site which accumulates post mortem in the study organism’s remains. Two simple and cost-effective methods to enrich the relative endogenous fraction of DNA in ancient samples involve treatment of sample powder with either bleach or Proteinase K pre-digestion prior to DNA extraction. Both approaches have yielded promising but varying results in other studies. Here, we contribute data on the performance of these methods using a comprehensive and systematic series of experiments applied to a single ancient bone fragment from a giant panda (Ailuropoda melanoleuca). SN - 1756-0500 UR - https://doi.org/10.1186/s13104-017-3061-3 DO - 10.1186/s13104-017-3061-3 ID - Basler2017 ER -