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Fig. 3 | BMC Research Notes

Fig. 3

From: Activation of MAP kinases by green leaf volatiles in grasses

Fig. 3

GLV generated from other plant species activated the Lt 46 and 44 kDa MAPKs. Three-four week-old Lt plants at the two tiller stage were placed into a closed clear (non-air tight) cylinder with freshly cut and damaged leaf tissue from plants indicated in the figure. One plant per time point was collected at the times indicated. Note control treatment plants were placed into the cylinders without any grass clippings present—see Fig. 1. To determine MAPK activity, immunoblots of protein extracts were performed using anti-phospho-MAPK (Erk1/2) antibody. The experiments were independently repeated three times. Representative blots are presented

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