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Table 1 Characterisation of 24 new L. malachurum microsatellites

From: Identification of 24 new microsatellite loci in the sweat bee Lasioglossum malachurum (Hymenoptera: Halictidae)

Locus name

GenBank sequence accession number

Panel and dye

Repeat motif

Primer sequence (5′–3′)

Na tested

N alleles

Expected allele sizeb, size range

HObs

HExp

HWE p value

Est. F (null)

L. cal successc

Lma02

MG273262

1

(TC)13

F: CCGAGTTCATCAACATCCTC

23

10

150

0.87

0.83

0.712

− 0.037

P

  

NED

R: TTGATTATCAGCGAGATGAGC

  

139–185

     

Lma03

MG273263

1

(AG)14

F: AAAGCGTTGCGAGACACC

38

7

154

0.816

0.745

0.103

− 0.063

P

  

PET

R: AGCATAATGGAAACCCAACG

  

137–167

     

Lma04

MG273264

 

(TG)12

F: CGTTACCGCGTTGGTTTC

37

6

169

0.649

0.727

0.162

0.034

M

   

R: GTCTTGTCTAACCGCAACAGC

  

165–177

     

Lma12

MG273265

2

(CT)12

F: CCAACCGAACACCAACTTTC

39

10

150

0.667

0.701

0.413

0.017

P

  

PET

R: CTCCCGGGTTGTCATGTAAG

  

131–181

     

Lma14

MG273266

1

(AG)14

F: CAACGCGTGACAGGTGATAC

40

14

170

0.825

0.896

0.186

0.035

P

  

6-FAM

R: CGGCTACGTTCCACTATGAAG

  

162–192

     

Lma20

MG273267

 

(AG)19

F: AGCGCTCGATGACTGTCG

39

17

210

0.872

0.889

0.087

0.007

F

   

R: TTGCGCAAGCCGTTCTAC

  

196–262

     

Lma21

MG273268

2

(GA)16

F: CGGTAAACTTGCTTCGACCTG

38

11

137

0.868

0.85

0.053

− 0.026

F

  

NED

R: CCGATTCCTTCACAGACACG

  

135–156

     

Lma23

MG273269

 

(GA)13

F: GATAATCAATGGTAATCGGTTGG

40

11

167

0.85

0.838

0.179

− 0.017

M

   

R: TTAACATCGTTCGCTTCTCG

  

154–218

     

Lma24

MG273270

2

(GA)13 CA (GA)6

F: TCCTCGGACAAGGAGATACG

40

13

172

0.925

0.891

0.723

− 0.026

P

  

6-FAM

R: TTCGGGTACCGTTCAGTCTC

  

141–181

     

Lma27

MG273271

 

(GA)13

F: GCTGGCAGCTCTGGAGAAG

38

9

189

0.737

0.804

0.071

0.032

P

   

R: TGACGGCCATTTAGTTCGTC

  

177–199

     

Lma29

MG273272

1

(CT)4 TT (CT)9

F: CTCGTCCCTCGTGTGACTC

38

12

204

0.868

0.883

0.725

0.003

P

  

PET

R: GTATCGTGCGTGCGTGTC

  

201–231

     

Lma30

MG273273

 

(GACGA)6

F: TCCGTCTCTGGTCGATACTG

38

3

237

0.447

0.407

0.854

− 0.075

P

   

R: ACAGCAGCATCTGAACTTGC

  

225–235

     

Lma31

MG273274

 

(TCTT)10

F: CGCACTCCGCTTTTCCTC

40

6

146

0.55

0.664

0.049

0.084

P

   

R: CGTCACCAGGAGAGCAAGG

  

142–164

     

Lma34

MG273275

 

(CT)12

F: TCTGAACAGTACGGAACAATGC

40

6

176

0.675

0.684

0.718

− 0.009

P

   

R: ACCGACACGGGAGAGAGAG

  

165–179

     

Lma36

MG273285

1

(CT)16

F: GGCCCTTCGACTTTGTTG

38

8

188

0.737

0.785

0.298

0.027

P

  

VIC

R: GAATCTCTGGGTGCTCTAACG

  

185–199

     

Lma39

MG273276

2

(CTAT)8

F: CGAGCCTATGCAGAGAACAG

38

7

205

0.789

0.75

0.68

− 0.034

P

  

PET

R: TGGATGGCTGCTGAGTAAAC

  

205–237

     

Lma40

MG273277

2

(GA)12

F: CGTTCGTTCGTTCGTTACTG

38

14

150

0.947

0.906

0.74

− 0.029

P

  

VIC

R: CAGAGTGCGTCGCTTGTTAG

  

155–189

     

Lma42

MG273278

 

(AG)13

F: ACCATCGCCCTTCCACTAC

40

5

167

0.75

0.733

0.623

− 0.016

P

   

R: CCGAAACTATTCGCCCATC

  

161–169

     

Lma48

MG273279

2

(TC)14

F: GTTGGATGCATCTGGAGGAC

38

6

206

0.763

0.722

0.14

− 0.043

M

  

NED

R: TGCGGTGGTTATTGATTTCC

  

193–209

     

Lma49

MG273280

 

(GAAA)10

F: GAGAGGGTGGTTGCACTACG

38

4

209

0.684

0.62

0.786

− 0.055

M

   

R: CTCGTGGAATCGAACTCACC

  

189–209

     

Lma50

MG273281

 

(CT)3 CG (CT)12

F: CGTTTAACCGGCTCGCTAC

38

8

181

0.684

0.763

0.726

0.051

P

   

R: CCGCGAATAAGTGGAGTGTC

  

163–209

     

Lma51

MG273282

1

(CT)11

F: GAGAAATTGCCAGCAAACATC

40

4

243

0.475

0.545

0.259

0.066

P

  

6-FAM

R: AGTTTCGTGGAAGGGAACG

  

237–243

     

Lma52

MG273283

1

(TG)11

F: CGGCAACTGCTTGCATAAC

40

5

156

0.8

0.732

0.575

− 0.056

M

  

VIC

R: CCCGTAGCACTCGCATACTC

  

151–159

     

Lma53

MG273284

1

(AC)12

F: ACGCGGGATTACTTTCAATC

40

9

228

0.675

0.759

0.053

0.057

P

  

NED

R: CCAATTATCGGGTGAAGGAG

  

217–241

     
  1. aN: number of diploid, unrelated L. malachurum females genotyped (all from the same population at Denton)
  2. bBased on the sequenced individual (sample M4); Hobs and HExp: observed and expected heterozygosities; HWE: p value when testing for deviation from Hardy–Weinberg equilibrium; F(Null): Estimated frequency of null alleles
  3. cAmplification success across 14 L. calceatum individuals: F failed to amplify, M monomorphic, p polymorphic