Table 2 Identification of a further 29 markers that were rejected and not considered for multiplex panels
Locus name | GenBank sequence accession number | Repeat motif | Primer sequence | Expected allele size† | Reason for dropping (tested in 23–24 individuals) |
|---|---|---|---|---|---|
Lma01 | MG273287 | (TGAC)7 | F: AACGCCTCGGTGAACCTG | 108 | Monomorphic |
R: TCGAGTTCTCCCTCCTCGTATC | |||||
Lma05 | MG273288 | (TTTC)7 | F: ATGCGTCTAAATCGTTCCTG | 178 | Monomorphic |
R: AACAAAGAATGAACGAACGTG | |||||
Lma06 | MG273289 | (AG)11 | F: CGGGAACGACGGAGAGAG | 184 | False peaks |
R: ACGGGTCTGTTCACCCTTTG | |||||
Lma07 | MG273286 | (GAAA)5 | F: GTCATGGAGAGGGTGGTTG | 189 | No product |
R: CAATCTCAACCGTGTTCGTC | |||||
Lma08 | MG273290 | (TTCT)7 | F: CTATCCGAGGCCTGTACACTG | 192 | No product |
R: ATCTGAAATCGTGGCTGGTC | |||||
Lma09 | MG273291 | (AGAA)5 | F: ACGGGACTGAAAGGGACAC | 201 | Monomorphic |
R: TACTTCGCGTGCCTGTCTC | |||||
Lma10 | MG273292 | (AAAG)7 | F: GAGACAACGAGGGAGAAAGC | 206 | Stutter |
R: AACCTCAACCGTGTTCGTC | |||||
Lma11 | MG273293 | (GA)7 | F: CTTGTACCACGCGTACACACC | 111 | False peaks |
R: GCCCTGCGTCTTCTCCTC | |||||
Lma13 | MG273294 | (CT)18 | F: GCTCATCGAGGACGAGGTG | 154 | False peaks |
R: GCGGTTGGCTGTCATAAGTG | |||||
Lma15 | MG273295 | (TGCT)5 | F: GGACAGTCCGACGAAGGAG | 179 | No product |
R: GCTTCATCCCTTTACTCCATAGC | |||||
Lma16 | MG273296 | (TC)20 | F: ACATTGTTCACCGGACAAATC | 187 | Monomorphic |
R: CGTCGAGGATAAGGTTACGG | |||||
Lma17 | MG273297 | (TC)11 | F: GTCAACGGTAATCCGAGGTG | 189 | False peaks/Stutter |
R: TGATACACCGGGAACCATTC | |||||
Lma18 | MG273298 | (AG)16 | F: GGGATACTAGACAGCCGGAATATAG | 193 | False peaks |
R: GAATGAACCACGCCGAAG | |||||
Lma19 | MG273299 | (TC)20 | F: TGTAAACGGCCGAAGTGTC | 203 | False peaks/Stutter |
R: ACAATGTGTGTTCCGGTCAG | |||||
Lma22 | MG273300 | (TCTT)5 | F: GCCGGACCAGATTAAATGC | 151 | No product |
R: AAAGACGAGGCTCAAAGAAGC | |||||
Lma25 | MG273301 | (CTAT)6 | F: CGAAATACCGTTAACCAACATC | 180 | Monomorphic |
R: TAAAGTGGCGAGTGATGGAC | |||||
Lma26 | MG273302 | (AG)16 | F: CTTCGATTCCTCGGGTCAC | 188 | No product |
R: TTCCGGCACGTTTATGTAGC | |||||
Lma28 | MG273303 | (CT)17 | F: ATTCGCGACAATGAACGAG | 193 | False peaks |
R: CAAACGCGAGTCAATAAATCC | |||||
Lma32 | MG273304 | (TC)16 | F: CGACGTACCTCTGCTTCCTC | 152 | Stutter |
R: AGGTCACTTAAATGGTGGTTGG | |||||
Lma33 | MG273305 | (GA)19 | F: CTCTTCTCGATTCCGTCTGG | 167 | False peaks |
R: TTTCGGCTCTTTGCTCTCTC | |||||
Lma35 | MG273306 | (GAGT)5 | F: CCTTCGAGAGGTCAGAGCTAAAG | 181 | No product |
R: CACGTGGCACCACAAATTC | |||||
Lma37 | MG273307 | (TTCT)5 | F: GTGGCCTATGCTCCTCTCC | 190 | Monomorphic |
R: ATCTGAAATCGTGGCTGGTC | |||||
Lma38 | MG273308 | (GACA)9 | F: AGAGACAAAGGCGGAGACAG | 197 | False peaks/Stutter |
R: TATCTGCGAGACCGACGA | |||||
Lma41 | MG273309 | (TC)20 | F: AATGATTGTGAACAGTTTGGTATG | 152 | Stutter |
R: CGAGACTGCAAGAAGTTTCAC | |||||
Lma43 | MG273310 | (AG)17 | F: TTCAGCCGAGGGTAGCAC | 178 | False peaks |
R: CGTACCATCATCTCGTGTCG | |||||
Lma44 | MG273311 | (AG)15 | F: ATGAGACTGGCACGACTGTG | 182 | False peaks |
R: ATGCGTCGCTCCCTTAATC | |||||
Lma45 | MG273312 | (CT)15 | F: TTTCGCATCCATCTTCCTTC | 189 | False peaks/Stutter |
R: CGCGAATTTCGGTATCTTTC | |||||
Lma46 | MG273313 | (TCCT)5 | F: TCCCTTTACCTTCCTTTCTCG | 190 | Monomorphic |
R: TGCAACATTTGTACCGAACAG | |||||
Lma47 | MG273314 | (CTTT)5 | F: CTATCCGAGGCCTGTACACTG | 197 | Stutter |
R: GGGTAAGCAAGCATCGTTTC |