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Table 2 Identification of a further 29 markers that were rejected and not considered for multiplex panels

From: Identification of 24 new microsatellite loci in the sweat bee Lasioglossum malachurum (Hymenoptera: Halictidae)

Locus name GenBank sequence accession number Repeat motif Primer sequence Expected allele size Reason for dropping (tested in 23–24 individuals)
Lma01 MG273287 (TGAC)7 F: AACGCCTCGGTGAACCTG 108 Monomorphic
    R: TCGAGTTCTCCCTCCTCGTATC   
Lma05 MG273288 (TTTC)7 F: ATGCGTCTAAATCGTTCCTG 178 Monomorphic
    R: AACAAAGAATGAACGAACGTG   
Lma06 MG273289 (AG)11 F: CGGGAACGACGGAGAGAG 184 False peaks
    R: ACGGGTCTGTTCACCCTTTG   
Lma07 MG273286 (GAAA)5 F: GTCATGGAGAGGGTGGTTG 189 No product
    R: CAATCTCAACCGTGTTCGTC   
Lma08 MG273290 (TTCT)7 F: CTATCCGAGGCCTGTACACTG 192 No product
    R: ATCTGAAATCGTGGCTGGTC   
Lma09 MG273291 (AGAA)5 F: ACGGGACTGAAAGGGACAC 201 Monomorphic
    R: TACTTCGCGTGCCTGTCTC   
Lma10 MG273292 (AAAG)7 F: GAGACAACGAGGGAGAAAGC 206 Stutter
    R: AACCTCAACCGTGTTCGTC   
Lma11 MG273293 (GA)7 F: CTTGTACCACGCGTACACACC 111 False peaks
    R: GCCCTGCGTCTTCTCCTC   
Lma13 MG273294 (CT)18 F: GCTCATCGAGGACGAGGTG 154 False peaks
    R: GCGGTTGGCTGTCATAAGTG   
Lma15 MG273295 (TGCT)5 F: GGACAGTCCGACGAAGGAG 179 No product
    R: GCTTCATCCCTTTACTCCATAGC   
Lma16 MG273296 (TC)20 F: ACATTGTTCACCGGACAAATC 187 Monomorphic
    R: CGTCGAGGATAAGGTTACGG   
Lma17 MG273297 (TC)11 F: GTCAACGGTAATCCGAGGTG 189 False peaks/Stutter
    R: TGATACACCGGGAACCATTC  
Lma18 MG273298 (AG)16 F: GGGATACTAGACAGCCGGAATATAG 193 False peaks
    R: GAATGAACCACGCCGAAG   
Lma19 MG273299 (TC)20 F: TGTAAACGGCCGAAGTGTC 203 False peaks/Stutter
    R: ACAATGTGTGTTCCGGTCAG  
Lma22 MG273300 (TCTT)5 F: GCCGGACCAGATTAAATGC 151 No product
    R: AAAGACGAGGCTCAAAGAAGC   
Lma25 MG273301 (CTAT)6 F: CGAAATACCGTTAACCAACATC 180 Monomorphic
    R: TAAAGTGGCGAGTGATGGAC   
Lma26 MG273302 (AG)16 F: CTTCGATTCCTCGGGTCAC 188 No product
    R: TTCCGGCACGTTTATGTAGC   
Lma28 MG273303 (CT)17 F: ATTCGCGACAATGAACGAG 193 False peaks
    R: CAAACGCGAGTCAATAAATCC   
Lma32 MG273304 (TC)16 F: CGACGTACCTCTGCTTCCTC 152 Stutter
    R: AGGTCACTTAAATGGTGGTTGG   
Lma33 MG273305 (GA)19 F: CTCTTCTCGATTCCGTCTGG 167 False peaks
    R: TTTCGGCTCTTTGCTCTCTC   
Lma35 MG273306 (GAGT)5 F: CCTTCGAGAGGTCAGAGCTAAAG 181 No product
    R: CACGTGGCACCACAAATTC   
Lma37 MG273307 (TTCT)5 F: GTGGCCTATGCTCCTCTCC 190 Monomorphic
    R: ATCTGAAATCGTGGCTGGTC   
Lma38 MG273308 (GACA)9 F: AGAGACAAAGGCGGAGACAG 197 False peaks/Stutter
    R: TATCTGCGAGACCGACGA  
Lma41 MG273309 (TC)20 F: AATGATTGTGAACAGTTTGGTATG 152 Stutter
    R: CGAGACTGCAAGAAGTTTCAC   
Lma43 MG273310 (AG)17 F: TTCAGCCGAGGGTAGCAC 178 False peaks
    R: CGTACCATCATCTCGTGTCG   
Lma44 MG273311 (AG)15 F: ATGAGACTGGCACGACTGTG 182 False peaks
    R: ATGCGTCGCTCCCTTAATC   
Lma45 MG273312 (CT)15 F: TTTCGCATCCATCTTCCTTC 189 False peaks/Stutter
    R: CGCGAATTTCGGTATCTTTC  
Lma46 MG273313 (TCCT)5 F: TCCCTTTACCTTCCTTTCTCG 190 Monomorphic
    R: TGCAACATTTGTACCGAACAG   
Lma47 MG273314 (CTTT)5 F: CTATCCGAGGCCTGTACACTG 197 Stutter
    R: GGGTAAGCAAGCATCGTTTC   
  1.  Based on the sequenced individual (sample M4)