Table 2 Ct values observed after qPCR amplification of Gemmata sp. DNA extracted from mock-infected blood culture bottles
From: Methods for detecting Gemmata spp. bacteremia in the microbiology laboratory
Initial concentration (bacteria/mL) | Gemmata massiliana Incubation: 7 days | Gemmata obscuriglobus Incubation: 7 days | Gemmata massiliana Incubation: 14 days | Gemmata obscuriglobus Incubation: 14 days | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
101 | 102 | 103 | 104 | 101 | 102 | 103 | 104 | 101 | 102 | 103 | 104 | 101 | 102 | 103 | 104 | |
qPCR results (CT) | 38.1 | 35.7 | – | 36.1 | – | – | 35.6 | – | – | – | 33.7 | – | – | – | – | |
– | – | – | 35.0 | – | – | 36.9 | 38.3 | – | – | – | 35.1 | – | – | – | 38.3 | |
– | 35.5 | 36.2 | 33.2 | – | – | – | – | – | – | – | 36.0 | – | – | – | 37.4 | |
– | – | 34.1 | 33.6 | – | 38.1 | 37.0 | – | – | – | – | 32.1 | – | – | – | – | |