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Table 2 Ct values observed after qPCR amplification of Gemmata sp. DNA extracted from mock-infected blood culture bottles

From: Methods for detecting Gemmata spp. bacteremia in the microbiology laboratory

Initial concentration (bacteria/mL) Gemmata massiliana
Incubation: 7 days
Gemmata obscuriglobus
Incubation: 7 days
Gemmata massiliana
Incubation: 14 days
Gemmata obscuriglobus
Incubation: 14 days
101 102 103 104 101 102 103 104 101 102 103 104 101 102 103 104
qPCR results (CT) 38.1 35.7 36.1 35.6   33.7
35.0 36.9 38.3 35.1 38.3
35.5 36.2 33.2 36.0 37.4
34.1 33.6 38.1 37.0 32.1
  1. –, no amplification detected; CT, cycle threshold
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