Fig. 2

Relative expression of Panx1, Panx2 and Panx3 mRNAs in undifferentiated hCBiPS2, HSC-1285 and HES-3 stem cell lines, as determined using quantitative real time PCR. The mean Ct of the reference gene was subtracted from the mean Ct of the gene of interest (ΔCt). Expression was then normalized to Panx1 (relative expression = 1; ΔΔCt) Relative mRNA expression was calculated as 2^−ΔΔCt according to Pfaffl [25]. In comparison, Panx1 showed the highest expression in all of the stem cell lines: a in hCBiPS2 cells, Panx2 expression was 8.26-fold lower and Panx3 expression was 6.25-fold lower than Panx1 expression; b in HSC-1285 cells, Panx2 expression was 12.82-fold lower and Panx3 expression was 8.06-fold lower as compared to Panx1; c in HES-3 cells, Panx2 expression was 7.75-fold lower and Panx3 expression was 2.98-fold lower than Panx1 expression. ***p < 0.0001