Fig. 3

Relative gene expression of Panx1, Panx2 and Panx3 in undifferentiated and differentiated cells, as determined via quantitative real time PCR. The mean Ct of the reference gene was subtracted from the mean Ct of the gene of interest (ΔCt). Expression was then normalized to the values obtained for undifferentiated cells. Relative mRNA expression was calculated as 2^−ΔΔCt according to Pfaffl [25]. a In hCBiPS2 cells, relative expression of Panx1 was 1.25-fold higher after undirected differentiation, whereas the relative expression of Panx2 was 2.374-fold higher and Panx3 was 2.373-fold higher in differentiated cells compared to undifferentiated cells. b In HSC-1285 cells the relative expression of Panx1 was 1.6-fold lower after undirected differentiation, whereas Panx2 was 2.922-fold higher and Panx3 was 1.242-fold higher in differentiated cells compared to undifferentiated cells; c in HES-3 cells the relative gene expression of Panx1 was 1.22-fold lower after undirected differentiation, whereas Panx2 was 2.39-fold higher and Panx3 was 1.17 lower in undifferentiated cells compared to undifferentiated cells. In hCBiPS2 cells, HSC-1285 cells, and in HES-3 cells, expression of Panx2 was significantly higher in differentiated cells as compared to undifferentiated cells. In hCBiPS2 cells Panx3 expression was significantly higher in differentiated cells as compared to undifferentiated cells. **p < 0.001; ***p < 0.0001