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Fig. 1 | BMC Research Notes

Fig. 1

From: A rapid high-resolution method for resolving DNA topoisomers

Fig. 1

Separation of topoisomers of plasmid pBR322 by agarose gel electrophoresis and QIAxcel Advanced System. a 1% agarose gel, stained with EtBr, of plasmid pBR322 relaxed by human topo I in the presence of the following concentrations of EtBr (µg/mL): A1–0; A2–0.002; A3–0.004; A4–0.006; A5–0.008; A6–0.010; A7–0.012; A8–0.014; A9–0.016; A10–0.02. The same samples were resolved by the QIAxcel system and are depicted as a digital gel image (b) and an electropherogram (c); colours in (b) match those in (c). d Individual densitometric trace of sample A7 from (c) illustrating the resolution of the topoisomers

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