Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection

Table 1 Identity of isolates determined using the algorithm

S/N	Information prior this study		Results of this study
	Previously determined ID	Species	WHO 2015	Species Resolving Modification (SRM)		Poliovirus Resolving Modification (PRM)			Final summary of isolates identified
	Previously determined ID	Species	AN89	189	187	Sab 1	Sab 2	Sab 3	Final summary of isolates identified
1	E3	EV-B	E3	unx	E3	Neg	Neg	Neg	E3
2	E19	EV-B	E7	unx	E19	Neg	Neg	Neg	E7/E19
3	E7	EV-B	E7	unx	E7	Neg	Neg	Neg	E7
4	E19	EV-B	Neg	E20	Neg	Neg	Neg	Neg	E20/E19
5	E7	EV-B	E7	Neg	E7	Neg	Neg	Neg	E7
6	E19	EV-B	Neg	unx	E19	Neg	Neg	Neg	E19
7	E7	EV-B	E7	unx	E7	Neg	Neg	Neg	E7
8	UNT	EV-B	E7	unx	E7	Neg	Neg	Neg	E7
9	CV-A13	EV-C	CV-A13	unx	CV-A13	Neg	Neg	Neg	CV-A13
10	CV-A13	EV-C	CV-A13	CV-A13	CV-A13	Neg	Neg	Neg	CV-A13
11	CV-A13	EV-C	CV-A13	CV-A13	unx	Neg	Neg	Neg	CV-A13
12	CV-A13	EV-C	CV-A13	CV-A13	Neg	Neg	Neg	Neg	CV-A13
13	PV-1	EV-C	PV-1	PV-1	PV-1	PV-1	PV-2	PV-3	PV-1, PV-2, PV-3
14	PV-2	EV-C	PV-2	PV-2	unx	Neg	PV-2^a	unx	PV-2
15	PV-3	EV-C	PV-3	PV-3	unx	Neg	Neg	PV-3^b	PV-3
16	PV-1, PV-2, PV-3, CV-A13 (S/N 11), E7 (S/N 3), E3 (S/N 1)	EV-B and C	PV-2	unx	PV-1	PV-1	PV-2^a	PV-3^b	PV-1, PV-2, PV-3

The primers included in the Species and Poliovirus Resolving Modifications (SRMs and PRMs) are referred to in the text as Species and Poliovirus Resolving Primers (SRPs and PRPs), respectively
The PRM did not amplify any of the isolates in samples 1–12
E Echovirus, EV enterovirus, PV Poliovirus, CV Coxsackievirus; unx unexploitable due to bad sequence data, Unt untypable, Neg negative (no amplification)
^{a, b}Isolates are genetically the same

ISSN: 1756-0500