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Fig. 3 | BMC Research Notes

Fig. 3

From: Neuroprotection by chitosan nanoparticles in oxidative stress-mediated injury

Fig. 3

Neuroprotection by Chi-DSNPs on H2O2 Challenged BV-2 Cells. a BV-2 cells were incubated with 0.1 mg/ml Chi-DSNPs of different sizes immediately or 15 min after challenging with 5500 μM H2O2. The data in H2O2 treated groups was normalized and compared with medium–medium treated group (***P < 0.001). The administration of 5 μm filtered group Chi-DSNPs, both at 0 min and post 15 min, enhanced cell viability significantly compared with H2O2-medium treated group (^^P < 0.01). Trypan blue was used to detect dead cells. All data was represented mean ± SD. b BV-2 cells were pre-incubated with 0.2 mg/ml Chi-DSNPs (pre-filtered with 1.2 or 5 μm syringe filter) for 4 h and then challenged with 50 μM H2O2 for 20 h. Cell proliferation was detected by WST assay. Data represented as mean ± SD. Cell proliferation was inhibited significantly by long-exposure of H2O2. An increasing of cell proliferation was observed in H2O2 challenged cells treated with 1.2 μm filtered Chi-DSNPs group (1.2 μm-NPs), and no difference was detected between injured control and Chi-DSNPs treatment (*P < 0.05, **P < 0.01, ***P < 0.001)

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