Skip to main content
Fig. 2 | BMC Research Notes

Fig. 2

From: Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae

Fig. 2

Presence of the CsR protein and cellular expression in C. subellipsoidae C-169. The TCL of C. subellipsoidea and two other closely related Chlorella vulgaris strain were probed with a pre-immune serum (Dilution; 1:3500), b KLH antibody (Dilution; 1:6000), with KLH protein loaded with TCLs, c western blot profile of TCL of C-169 probed with CsR antibody (Dilution; 1:3500). d Dimer profiling of CsR in C. ellipsoidea. The sample prepared in three denaturing reagents (SDS, DTT, βME) and incubated at three different temperatures and probed with anti CsRAb without denaturant (lane 1–3), with SDS (lane 4–6), with SDS and DTT (lane 7–9) and with SDS, DTT and βME (lane 10–12). The immunoblots are representative image of three different experimental sets

Back to article page