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Fig. 1 | BMC Research Notes

Fig. 1

From: Considerations for skin carcinogenesis experiments using inducible transgenic mouse models

Fig. 1

a Schematic representation of the keratin-14 (K14)-driven inducible Cre-recominase (Cre) and the KrasG12D and p53R172H transgenes prior the excision of lox-STOP-lox cassette. ßg int: Beta-globin 5’ untranslated region (UTR) and an intronic sequence. ERtam: tamoxifen inducible estrogen receptor. PolyA: polyadenylation signal. b K14-driven (red) expression in skin. c, d Transgenes after recombination (c) and schematic of tumour development and progression. e A 10 Gauge (g) trochar and tamoxifen pellet used for the surgeries. f Number of mice with a correct (red) and incorrect (grey) genotype after breeding to generate experimental cohorts. g Site of pellet insertion (left hand panel) and examples of successful pellet insertion (upper right hand panel) and a partially extruded pellet (lower right hand panel). h Number of mice showing pellet extrusion (red) in the experiment. i Weight of mice with extruded pellets and not extruded pellets (no complications). Lines indicate mean values

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