Fig. 3
From: Automated cell cycle and cell size measurements for single-cell gene expression studies
Analysis of the influence of the staining procedure on gene expression. a Real-time PCR gene expression analysis of stained and unstained cells. Total RNA was extracted from T2EC cells stained or not. Reverse transcription and real-time PCR analyses, with specific primers [21], were performed to quantify the amount of GLOBIN (\(\beta\)-GLOBIN), SLC (SLC25A37), HSP (HSP90AA1), CRIP2 and LDHA mRNA (\(C_q\) for cycle of quantification). The fold variations represented here correspond to the ratio of mRNA of staining cells compared to unstained cells. The black line corresponds to the null variation between the two conditions. The vertical bars represent the standard error of the mean value (n = 3). b Principal Component Analysis of single cell expression data acquired on stained or unstained cells. Projection of 77 T2EC single-cell stained or not onto PC1 and PC2 results in a cloud of points without any clear separation. Percentages shown are the percentage of variance explained by each component