Fig. 3

Evolutionary relationships of concatenated proteins encoded by eps gene clusters (a) and MLSA phylogenetic relationships between strains (b). Main monophyletic clades are also indicated in both trees. Concatenated proteins were aligned with COBALT and MLSA five-gene dataset was aligned with MAFFT. Phylogenetic trees were constructed with MEGA version7 using the Neighbor-Joining method [13, 14] with the Poisson correction method for proteins [38] and the Jukes Cantor substitution model for MLSA [39]. Bootstrap values (1000 replicates) are shown next to the branches when higher than 70 [40]