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Fig. 3 | BMC Research Notes

Fig. 3

From: Degenerate codon mixing for PCR-based manipulation of highly repetitive sequences

Fig. 3

Analysis of the Tol2-QX-GFP-v2A-GFP construct expression in D. rerio. Zebrafish embryos were injected with 25 ng/µL of the Tol2-QX-GFP-v2A-GFP construct and 25 ng/µL mRNA coding for Tol2 transposase. a Brightfield (top panels) and fluorescent (bottom panels) images of the left side view of zebrafish embryo head and trunk regions expressing Q80, Q52, Q21 and Q11-GFP, ~ 24 hpf. b Brightfield (top panels) and fluorescent (bottom panels) images of the left side view of zebrafish embryo trunk and tail regions expressing Q80, Q52, Q21 and Q11-GFP, ~ 24 hpf. c Western blot analysis of the expression of Qx-GFP constructs in D. rerio. Proteins were isolated from ~ 24 hpf embryos expressing the Q80, Q52, Q21 and Q11-GFP constructs. The proteins were resolved on a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane. The membrane was probed with an anti-GFP antibody. The “empty” Tol2 vector possesses an expressed GFP gene that is replaced during construct insertion. d Western blot quantification. The GFP intensity for each numbered band of the Western blot and the Qx-GFP to free GFP ratio are presented

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