Skip to main content

Advertisement

Table 1 List of primers and probes used in this study

From: Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

Organism Name Primers/probes Target region
Blastocystis sp. Blasto FWD F5 5′-GGTCCGGTGAACACTTTGGATTT-3′ 18S
Blasto R F2 5′-CCTACGGAAACCTTGTTACGACTTCA-3′  
Blasto probe 5′-FAM-TCGTGTAAATCTTACCATTTAGAGGA-MGBNFQ-3′  
Cryptosporidium parvum/hominis 1PS_F 5′-AACTTTAGCTCCAGTTGAGAAAGTACTC-3′ Hsp70 gene
1PS_R 5′-CATGGCTCTTTACCGTTAAAGAATTCC-3′  
Crypt_P 5′-FAM-AATACGTGTAGAACCACCAACCAATACAACATC-TAMRA-3′  
Cyclospora cayetanensis Cyclo250F 5′-TAGTAACCGAACGGATCGCATT-3′ 18S
Cyclo350R 5′-AATGCCACGTAGGCCAATA-3′  
Cyclo281T 5′-FAM-CCGGCGATAGATCATTCAAGTTTCTGACC-TAMRA-3′  
Cystoisospora belli Ib-40F 5′-ATATTCCCTGCAGCATGTCTGTTT-3′ ITS2
Ib-129R 5′-CCACACGCGTATTCCAGAGA-3′  
Ib-81Taq 5′-FAM-CAAGTTCTGCTCACGCGCTTCTGG-TAMRA-3′  
Dientamoeba fragilis Df-124F 5′-CAACGGATGTCTTGGCTCTTTA-3′ 18S
Df-221R 5′-TGCATTCAAAGATCGAACTTATCAC-3′  
Df-172revT 5′-VIC CAATTCTAGCCGCTTAT-BHQ1-3′  
Enterocytozoon bieneusi FEB1 5′-CGCTGTAGTTCCTGCAGTAAACTATGCC-3′ 18S
REB1 5′-CTTGCGAGCGTACTATCCCCAGAG-3′  
PEB1 5′-FAM-ACGTGGGCGGGAGAAATCTTTAGTGTTCGGG-TAMRA-3′  
Giardia intestinalis Giardia-80F 5′-GACGGCTCAGGACAACGGTT-3′ 18S
Giardia-127R 5′-TTGCCAGCGGTGTCCG-3′  
Giardia-105T 5′-FAM-CCCGCGGCGGTCCCTGCTAG-BHQ1-3′  
TTB (all bacteria) TTB_16S_F 5′- AGAGTTTGATCMTGGCTCAG-3′ 16S
TTB_16S_R 5′- TTACCGCGGCKGCTGGCAC-3′  
TTB338K_FAM 5′-FAM- CCAKACTCCTACGGGAGGCAGCAG-3′