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Table 1 List of primers and probes used in this study

From: Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

Organism

Name

Primers/probes

Target region

Blastocystis sp.

Blasto FWD F5

5′-GGTCCGGTGAACACTTTGGATTT-3′

18S

Blasto R F2

5′-CCTACGGAAACCTTGTTACGACTTCA-3′

 

Blasto probe

5′-FAM-TCGTGTAAATCTTACCATTTAGAGGA-MGBNFQ-3′

 

Cryptosporidium parvum/hominis

1PS_F

5′-AACTTTAGCTCCAGTTGAGAAAGTACTC-3′

Hsp70 gene

1PS_R

5′-CATGGCTCTTTACCGTTAAAGAATTCC-3′

 

Crypt_P

5′-FAM-AATACGTGTAGAACCACCAACCAATACAACATC-TAMRA-3′

 

Cyclospora cayetanensis

Cyclo250F

5′-TAGTAACCGAACGGATCGCATT-3′

18S

Cyclo350R

5′-AATGCCACGTAGGCCAATA-3′

 

Cyclo281T

5′-FAM-CCGGCGATAGATCATTCAAGTTTCTGACC-TAMRA-3′

 

Cystoisospora belli

Ib-40F

5′-ATATTCCCTGCAGCATGTCTGTTT-3′

ITS2

Ib-129R

5′-CCACACGCGTATTCCAGAGA-3′

 

Ib-81Taq

5′-FAM-CAAGTTCTGCTCACGCGCTTCTGG-TAMRA-3′

 

Dientamoeba fragilis

Df-124F

5′-CAACGGATGTCTTGGCTCTTTA-3′

18S

Df-221R

5′-TGCATTCAAAGATCGAACTTATCAC-3′

 

Df-172revT

5′-VIC CAATTCTAGCCGCTTAT-BHQ1-3′

 

Enterocytozoon bieneusi

FEB1

5′-CGCTGTAGTTCCTGCAGTAAACTATGCC-3′

18S

REB1

5′-CTTGCGAGCGTACTATCCCCAGAG-3′

 

PEB1

5′-FAM-ACGTGGGCGGGAGAAATCTTTAGTGTTCGGG-TAMRA-3′

 

Giardia intestinalis

Giardia-80F

5′-GACGGCTCAGGACAACGGTT-3′

18S

Giardia-127R

5′-TTGCCAGCGGTGTCCG-3′

 

Giardia-105T

5′-FAM-CCCGCGGCGGTCCCTGCTAG-BHQ1-3′

 

TTB (all bacteria)

TTB_16S_F

5′- AGAGTTTGATCMTGGCTCAG-3′

16S

TTB_16S_R

5′- TTACCGCGGCKGCTGGCAC-3′

 

TTB338K_FAM

5′-FAM- CCAKACTCCTACGGGAGGCAGCAG-3′