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Fig. 1 | BMC Research Notes

Fig. 1

From: Rescue of an aggressive female sexual courtship in mice by CRISPR/Cas9 secondary mutation in vivo

Fig. 1

Inactivating mutation in HOXD10 ectopically expressed in a minor GABAergic subpopulation in adult HoxDAfc brain. a Comparison of wild type HoxD and the HoxDAfc alias Del(19) and HoxDDel(19)d10hd mutant alleles. Discontinuity of the horizontal line indicates the absence of the genomic segment and the red X indicates the position of the CRISPR/Cas9 hit in the Hoxd10 homeobox, leading to the generation of the HoxDDel(19)d10hd allele. b Amino-acid sequence of the HOXD10 homeodomain in both the wild type and the HoxDAfc alleles. The three alpha helical subdomains are underlined. c Amino-acid sequence of the homeodomain in the truncated HOXD10hd protein product. The sequence of the remaining two alpha helical subdomains are underlined and an asterisk indicates an out of frame stop codon. d, e Details of representative coronal sections of heterozygous HoxDAfc female brains. The contours of the hippocampal formation are indicated by red dots and three landmark cytoarchirectonic layers are annotated (sr, sp, so, for strata radiatum, pyramidale and oriens, respectively). d Gad1 specific antisense probe reveals positive cells distributed in all layers of Cornu Ammonis (CA). e A Cck specific antisense probe shows few strongly stained cells in all layers of CA, and a relatively weaker signal in the rest of the cells located in sp. f, g, h Simultaneous fluorescent in situ hybridization. Nuclei are shown in blue. f Expression of Cck in green (top) is detected in one of four Gad1 positive cells shown below in red (bottom) in CA3 sr. g Expression of Hoxd10 (red, top) in one of four Gad1 positive cells (green, bottom) in CA3 so. h Expression of Hoxd10 in (red, top) in one of the three Cck positive cells (green, bottom) in CA1 so

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