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Fig. 1 | BMC Research Notes

Fig. 1

From: A multi-host approach to identify a transposon mutant of Pseudomonas aeruginosa LESB58 lacking full virulence

Fig. 1

Example of a non-virulent LESB58 STM mutant in the amoeba model. Drops of determined amoeba concentrations were spotted on a bacterial lawn, allowing a semi-quantitative determination of the bacteria’s predation resistance after 6 days of incubation at 21–23 °C. Phagocytic plaques appear as light areas on the bacterial lawn. At least 1000 D. discoideum cells/5 μL were required to pierce the wild-type bacterial lawn, whereas fewer amoeba cells were needed to form a phagocytic plaque for mutants with reduced virulence in this context. The results were later confirmed (n = 3)

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