Storage time (weeks) | The number of M. bovis (CFU/mL) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
4 °C | − 20 °C | − 80 °C | ||||||||||
CONa | FBSb | GELc | GLYd | CON | FBS | GEL | GLY | CON | FBS | GEL | GLY | |
1 | 301.7 ± 94.5 | 327.6 ± 97.4 | 361.9 ± 100 | 312 ± 101 | 304.7 ± 93.7 | 342.8 ± 99.5 | 323.8 ± 96.3 | 320 ± 100.7 | 316.1 ± 106.9 | 327.6 ± 58.1 | 342.8 ± 100.5 | 320 ± 100.9 |
2 | 285.3 ± 95.8 | 300.9 ± 92 | 369.5 ± 99 | 281 ± 91 | 281.9 ± 90.6 | 335.2 ± 94.9 | 327.6 ± 91.0 | 308.5 ± 96.1 | 204.2 ± 90.8 | 266.6 ± 58.5 | 300.9 ± 100.1 | 350.4 ± 105.9 |
4 | 154.6 ± 23.3 | 194 ± 64.3 | 388 ± 102 | 300 ± 96 | 262.8 ± 85.9 | 274.2 ± 79.7 | 300.9 ± 110.3 | 297.1 ± 93.8 | 117.1 ± 43.8 | 240 ± 63.8 | 281.9 ± 89.1 | 380.9 ± 98.7 |
8 | 56.5 ± 23.8 | 125.7 ± 46 | 201.9 ± 75.3 | 289.5 ± 95 | 137.1 ± 74.9 | 148.5 ± 52.4 | 262.8 ± 110.0 | 335.2 ± 103.3 | 38 ± 20.9 | 91.4 ± 20.9 | 224.7 ± 96.8 | 384.7 ± 102.3 |
16 | 16.6 ± 11.4 | 72.3 ± 28.9 | 160 ± 66.6 | 186.6 ± 73.7 | 57.1 ± 34 | 83.8 ± 42.2 | 110.4 ± 52.8 | 251.4 ± 78.1 | 11.4 ± 6.1 | 38.1 ± 19.6 | 144.7 ± 83.6 | 266.6 ± 94.5 |