Skip to main content

Table 1 The mean values (± SE) of viable colonies of Mycoplasma bovis for 21 milk samples after storage at 4, − 20, or − 80 °C in milk or milk supplemented with Foetal Bovine Serum (40% v-v) + Dimethyl Sulphoxide (10% v-v), Gelatin (40% v-v) + DMSO (10% v-v) or Glycerol (40% v-v) + DMSO (10% v-v)

From: Evaluation of three cryoprotectants used with bovine milk affected with Mycoplasma bovis in different freezing conditions

Storage time (weeks)

The number of M. bovis (CFU/mL)

4 °C

− 20 °C

− 80 °C

CONa

FBSb

GELc

GLYd

CON

FBS

GEL

GLY

CON

FBS

GEL

GLY

1

301.7 ± 94.5

327.6 ± 97.4

361.9 ± 100

312 ± 101

304.7 ± 93.7

342.8 ± 99.5

323.8 ± 96.3

320 ± 100.7

316.1 ± 106.9

327.6 ± 58.1

342.8 ± 100.5

320 ± 100.9

2

285.3 ± 95.8

300.9 ± 92

369.5 ± 99

281 ± 91

281.9 ± 90.6

335.2 ± 94.9

327.6 ± 91.0

308.5 ± 96.1

204.2 ± 90.8

266.6 ± 58.5

300.9 ± 100.1

350.4 ± 105.9

4

154.6 ± 23.3

194 ± 64.3

388 ± 102

300 ± 96

262.8 ± 85.9

274.2 ± 79.7

300.9 ± 110.3

297.1 ± 93.8

117.1 ± 43.8

240 ± 63.8

281.9 ± 89.1

380.9 ± 98.7

8

56.5 ± 23.8

125.7 ± 46

201.9 ± 75.3

289.5 ± 95

137.1 ± 74.9

148.5 ± 52.4

262.8 ± 110.0

335.2 ± 103.3

38 ± 20.9

91.4 ± 20.9

224.7 ± 96.8

384.7 ± 102.3

16

16.6 ± 11.4

72.3 ± 28.9

160 ± 66.6

186.6 ± 73.7

57.1 ± 34

83.8 ± 42.2

110.4 ± 52.8

251.4 ± 78.1

11.4 ± 6.1

38.1 ± 19.6

144.7 ± 83.6

266.6 ± 94.5

  1. aControl (milk only); b Foetal Bovine Serum (40% v-v) + Dimethyl Sulphoxide (10% v-v); c Gelatin (40% v-v) + DMSO (10% v-v); d Glycerol (40% v-v) + DMSO (10% v-v)