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Table 1 The mean values (± SE) of viable colonies of Mycoplasma bovis for 21 milk samples after storage at 4, − 20, or − 80 °C in milk or milk supplemented with Foetal Bovine Serum (40% v-v) + Dimethyl Sulphoxide (10% v-v), Gelatin (40% v-v) + DMSO (10% v-v) or Glycerol (40% v-v) + DMSO (10% v-v)

From: Evaluation of three cryoprotectants used with bovine milk affected with Mycoplasma bovis in different freezing conditions

Storage time (weeks) The number of M. bovis (CFU/mL)
4 °C − 20 °C − 80 °C
CONa FBSb GELc GLYd CON FBS GEL GLY CON FBS GEL GLY
1 301.7 ± 94.5 327.6 ± 97.4 361.9 ± 100 312 ± 101 304.7 ± 93.7 342.8 ± 99.5 323.8 ± 96.3 320 ± 100.7 316.1 ± 106.9 327.6 ± 58.1 342.8 ± 100.5 320 ± 100.9
2 285.3 ± 95.8 300.9 ± 92 369.5 ± 99 281 ± 91 281.9 ± 90.6 335.2 ± 94.9 327.6 ± 91.0 308.5 ± 96.1 204.2 ± 90.8 266.6 ± 58.5 300.9 ± 100.1 350.4 ± 105.9
4 154.6 ± 23.3 194 ± 64.3 388 ± 102 300 ± 96 262.8 ± 85.9 274.2 ± 79.7 300.9 ± 110.3 297.1 ± 93.8 117.1 ± 43.8 240 ± 63.8 281.9 ± 89.1 380.9 ± 98.7
8 56.5 ± 23.8 125.7 ± 46 201.9 ± 75.3 289.5 ± 95 137.1 ± 74.9 148.5 ± 52.4 262.8 ± 110.0 335.2 ± 103.3 38 ± 20.9 91.4 ± 20.9 224.7 ± 96.8 384.7 ± 102.3
16 16.6 ± 11.4 72.3 ± 28.9 160 ± 66.6 186.6 ± 73.7 57.1 ± 34 83.8 ± 42.2 110.4 ± 52.8 251.4 ± 78.1 11.4 ± 6.1 38.1 ± 19.6 144.7 ± 83.6 266.6 ± 94.5
  1. aControl (milk only); b Foetal Bovine Serum (40% v-v) + Dimethyl Sulphoxide (10% v-v); c Gelatin (40% v-v) + DMSO (10% v-v); d Glycerol (40% v-v) + DMSO (10% v-v)