Fig. 2
Hs578T/NOD1 and Hs578T/NOD2 populations highly express respective transgenes and display a lower proliferative rate. a Relative NOD1 and NOD2 transcripts detection by RT-qPCR in Hs578T cell populations after transduction with lentiviruses for overexpression of GFP, NOD1 or NOD2. Normalization was performed as indicated in Fig. 1 (mean + standard deviation, n = 3). b Ponceau S staining and anti-FLAG immunoblot detection of overexpressed GFP (arrowhead), NOD1 and NOD2 (arrow) proteins in transduced H578T populations. c Detection of GFP-positive cells in Hs578T populations overexpressing NOD genes by flow cytometry (FSC-A vs FITC). Positive cells are gated inside the upper right quadrant, representing over 97% of all transduced populations. d Growth curves comparing the proliferative rate of the wild-type versus transduced Hs578T populations overexpressing GFP, NOD1 or NOD2. 5x103 cells were seeded in 3.8 cm2 wells (12-well plate) and cultured for six days. Total cell number per well is presented at each time point (two-way ANOVA, n=3)