Fig. 2

Expression of intercellular adhesion molecule (ICAM)-1-related (ICR) and ICAM-1 by human retinal endothelial cells. Primary endothelial cells were individually isolated from retinae of four different human donors, and exposed to tumor necrosis factor (TNF)-α-supplemented or control medium for 4 h (n = 3 replicate cultures/condition). a Graph shows mean cycle threshold values obtained in quantitative real-time polymerase chain reaction for ICR and ICAM-1 cDNA. Results for each human donor are represented by a unique shape, and represent (TNF)-α-supplemented versus control values. b Graphs show relative expression of ICR and ICAM-1 transcript by human donor. Bars represent mean relative expression, with error bars showing standard error of the mean. Reference genes were β-actin and glyceraldehyde-3-phosphate dehydrogenase. Data were analyzed by two-tailed Student’s t test