Fig. 1

The purity of isolated hAECs isolated from the amnion membrane. a More than 97% of the isolated hAECs were positive for cytokeratin, an epithelial cell marker. b, c < 1% of the isolated cells were positive for MSC markers [CD90 (b) and CD105 (c)]. Blue shaded histogram: MSCs were stained with anti-CD90 (b) and anti-CD105 (c) antibodies as positive controls. Gray shaded histogram: hAECs were stained with matched-isotype control antibodies as negative controls (a–c). Black line: hAECs were stained with anti-cytokeratin (a), anti-CD90 (b) and anti-CD105 (c) antibodies. Data are representative of five independent experiments. All data show mean ± SEM