Skip to main content
Fig. 1 | BMC Research Notes

Fig. 1

From: Screening of melatonin, α-tocopherol, folic acid, acetyl-l-carnitine and resveratrol for anti-dengue 2 virus activity

Fig. 1

Effect of melatonin on DENV 2 infection of HEK293T/17 and HepG2 cells. The cytotoxicity of melatonin was assessed in a uninfected HEK293T/17 (left panel) and HepG2 (right panel) and b DENV 2 infected HEK293T/17 (left panel and HepG2 (right panel) cells. Infected and uninfected cells were incubated with various concentration of melatonin for 24 h and viability was assessed using MTT assay. The experiments were performed independently in triplicate in parallel with control treatments and mock. A negative of cells incubated with 5% DMSO in complete media was included. The standard deviation (SD) of mean are presented as error bars. c HEK293T/17 cells were infected with DENV 2 at MOI 0.5 and 2, and then treated with or without 50 and 500 µM of melatonin or with vehicle only. d HepG2 cells were infected with DENV 2 at MOI 2 and 5 and then treated with or without 50 and 500 µM of melatonin or with vehicle only. At 24 h post infection cells were analyzed by flow cytometry to determine the percentage infection. All experiments were undertaken independently in triplicate. Error bar showed mean ± SD (*p value ≤ 0.05)

Back to article page