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Table 1 Effect of in vivo passage of Chlamydia spp. stocks on Mycoplasma contamination

From: Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

Species (strain) route Isolates tested (mouse strain) Result, in vitro pass 1 Result, in vitro pass 2
MycoAlert™ PCRa MycoAlert™ PCRa
C. muridarum (Weiss strain) intravaginal Culture stockb + + +2 +
c-vc swab 1 (BALB/c) day 4 nd
c-v swab 2 (BALB/c) day 4 nd
c-v swab 3 (BALB/c) day 4 nd
c-v swab 4 (C3H/HeN) day 4 nd
c-v swab 5 (C3H/HeN) day 4 nd
c-v swab 6 (C3H/HeN) day 4 nd
C. trachomatis (serovar E/Bour) intravaginal Culture stockb + + + +
c-v swab 1 (C57BL/6) day 4
c-v swab 2 (C57BL/6) day 4
c-v swab 3 (C57BL/6) day 4
c-v swab 4 (C57BL/6) day 4
c-v swab 5 (C57BL/6) day 7
c-v swab 6 (C57BL/6) day 7
c-v swab 7 (C57BL/6) day 7
c-v swab 8 (C57BL/6) day 7
C. pneumoniae (TW-183) intranasal Culture stockb + + + +
Lung 1 (C57BL/6) day 4
Lung 2 (C57BL/6) day 4
Lung 3 (C57BL/6) day 4
Lung 4 (C57BL/6) day 4
Lung 5 (C57BL/6) day 7
Lung 6 (C57BL/6) day 7
Lung 7 (C57BL/6) day 7
Lung 8 (C57BL/6) day 7
  1. aPCR for GPO-3/MGSO positive with a PCR product band at the anticipated size of ~ 270 bp on agarose gel electrophoresis. A positive control for chlamydial 16 rRNA was included in each reaction
  2. bAll culture stocks of each of these strains repeatedly tested positive by MycoAlert™. In this assay, a before/after ratio of ATP of 1.0 or greater is considered a positive result by the manufacturer. All of our culture stocks were positive between 5.0 and 16.0 in multiple tests for each stock
  3. cc-v = cervical vaginal swab collected as previously described in Cotter et al. [9];
  4. nd not determined