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Table 2 Effect of plaque isolation assay on Mycoplasma contamination

From: Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

Plaque isolate numbera Result, passage 1 Result, passage 2
MycoAlert™ PCR MycoAlert™ PCR
1 b + + +
2 + + +
3 + + +
4 + +
5 + + +
6 + + +
7 + + +
8 + + +
9 +
10
11 + + +
12
13
14
15
16 + + + +
17
18
19
20
21
22
  1. aStock from which random plaques were selected was C. muridarum (Weiss strain) and were consistently positive by MycoAlert™
  2. bAll culture stocks of each of these strains repeatedly tested positive by MycoAlert™. In this assay, a before/after ratio of ATP of 1.0 or greater is considered a positive result by the manufacturer. All of our culture stocks were positive between 5.0 and 16.0 in multiple tests for each stock