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Table 2 Effect of plaque isolation assay on Mycoplasma contamination

From: Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

Plaque isolate numbera

Result, passage 1

Result, passage 2

MycoAlertâ„¢

PCR

MycoAlertâ„¢

PCR

1

−b

+

+

+

2

−

+

+

+

3

−

+

+

+

4

−

+

−

+

5

−

+

+

+

6

−

+

+

+

7

−

+

+

+

8

−

+

+

+

9

−

+

−

−

10

−

−

−

−

11

−

+

+

+

12

−

−

−

−

13

−

−

−

−

14

−

−

−

−

15

−

−

−

−

16

+

+

+

+

17

−

−

−

−

18

−

−

−

−

19

−

−

−

−

20

−

−

−

−

21

−

−

−

−

22

−

−

−

−

  1. aStock from which random plaques were selected was C. muridarum (Weiss strain) and were consistently positive by MycoAlertâ„¢
  2. bAll culture stocks of each of these strains repeatedly tested positive by MycoAlertâ„¢. In this assay, a before/after ratio of ATP of 1.0 or greater is considered a positive result by the manufacturer. All of our culture stocks were positive between 5.0 and 16.0 in multiple tests for each stock