Fig. 1From: Multiplex PCR for the simultaneous detection of the Enterobacterial gene wecA, the Shiga Toxin genes (stx1 and stx2) and the Intimin gene (eae)Triplex PCR optimization for MgCl2 concentration. Lanes 2–4 2 mM MgCl2, Lanes 5–7 3 mM MgCl2, Lanes 8–10 4 mM MgCl2, Lane 1 DNA size ladder, Lanes 2, 5 and 8 E. coli O157:H7, Lanes 3, 6 and 9 E. coli O157:H7 and S. dysenteriae, Lanes 4, 7 and 10 S. dysenteriae, Lane 11 negative controlBack to article page