Fig. 1

Detection of AGS3 in rat whole kidney and urinary exosomes. a Representative photomicrographs SD and PCK rat kidneys at 24 weeks are presented (original magnification, ×10). Circles indicate individual values. Line indicates group median. *P < 0.05. BW: body weight. TKW: total kidney weight. b Representative Western blot demonstrating AGS3 expression in SD and PCK rat kidneys at 8, 16, and 24 weeks of age. Rat brain was used as a positive control for AGS3. β-actin was used as a loading control. c Time course change in AGS3 protein in urine exosomes. SD and PCK rats were placed in metabolic cages to collect urine for 24 h at 8, 12, 16 and 20 weeks of age, and a Western blot of AGS3 using the proteins isolated from rat urinary exosomes is shown for SD rats (16 weeks) and PCK rats (8, 12, 16 and 20 weeks). d, e Urine exosome collection in rats and measurement of AGS3 protein by Western blot analysis. SD (n = 8) and PCK (n = 6) rats were placed in metabolic cages to collect urine for 24 h at 16 weeks of age. Urine samples were then centrifuged to isolate urinary exosomes. d The presence of urinary exosomes (arrows) in rat urine was confirmed by electron microscopy. Scale bar = 100 nm. e AGS3 protein expression in urinary exosomes from 16-week-old SD and PCK rat was measured by densitometry. Circles represent individual log2 transformed values. Line indicates group mean. *P < 0.05