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Fig. 2 | BMC Research Notes

Fig. 2

From: An enChIP system for the analysis of bacterial genome functions

Fig. 2

Isolation of an endogenous gene promoter by enChIP. a Positions of gRNAs in the rpoH promoter. Green highlight: gRNA 158–180; blue highlight: gRNA 184–206; red letters: ftsX gene coding sequence; green letters: rpoH gene coding sequence; underlined: protospacer adjacent motif (PAM) sequences. b Positions of gRNAs and primer sets targeting the rpoH gene. The positions of primer sets are shown in red. c Isolation of the rpoH gene promoter by enChIP. Real-time PCR analysis of chromatin complexes isolated by enChIP is shown. An irrelevant locus (lacZ) was analyzed as a negative control

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