Table 1 Oligodeoxyribonucleotides used in this study

27816

E. coli gRNA common S

agttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttga

Construction of plasmids targeting the rpoH gene promoter

27817

E. coli gRNA common A

agcttcaaaaaaagcaccgactcggtgccactttttcaagttgataacggactagcct

Construction of plasmids targeting the rpoH gene promoter

27818

rpoH p 158–180 S

ctagtgttatactctttccctgcaagttttagagctagaaatagca

Construction of the plasmid targeting the rpoH gene promoter 158–180

27819

rpoH p 158–180 A

tattttaacttgctatttctagctctaaaacttgcagggaaagagtataaca

Construction of the plasmid targeting the rpoH gene promoter 158–180

27820

rpoH p 184–206 S

ctagtcggggtctctttccctgctagttttagagctagaaatagca

Construction of the plasmid targeting the rpoH gene promoter 184–206

27821

rpoH p 184–206 A

tattttaacttgctatttctagctctaaaactagcagggaaagagaccccga

Construction of the plasmid targeting the rpoH gene promoter 184–206

27792

LacZ-E. coli-F

gcgattaccgttgatgttgaagt

Real-time PCR in Figs. 1d and 2d (LacZ)

27793

LacZ-E. coli-R

agtaaggcggtcgggatagtttt

Real-time PCR in Figs. 1d and 2d (LacZ)

27794

LacZ-E. coli-F2

aaaactatcccgaccgccttact

Real-time PCR in Fig. 1d (LacZ(2))

27795

LacZ-E. coli-R2

gggaagacgtacggggtatacat

Real-time PCR in Fig. 1d (LacZ(2))

27796

crp-E. coli-F

tcacttcagagaaagtgggcaac

Real-time PCR in Fig. 1d (crp)

27797

crp-E. coli-R

gtcatagcgtctggttgttttgc

Real-time PCR in Fig. 1d (crp)

27798

LacI-E. coli-F

cgtcagtgggctgatcattaact

Real-time PCR in Fig. 1d (LacI)

27799

LacI-E. coli-R

atcaagaaataacgccggaacat

Real-time PCR in Fig. 1d (LacI)

27902

rpoH-E. coli-F

aagcttgcattgaacttgtggat

Real-time PCR in Fig. 2d (rpoH-prom)

27903

rpoH-E. coli-R

tatcttctggcgcttcagtggta

Real-time PCR in Fig. 2d (rpoH-prom)

27896

rpoH-coding-F

tacgttctgcgtaactggcgtat

Real-time PCR in Fig. 2d (rpoH-cod)

27897

rpoH-coding-R

accatttcgacttcatcctggtt

Real-time PCR in Fig. 2d (rpoH-cod)