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Fig. 3 | BMC Research Notes

Fig. 3

From: The remarkable plethora of infestation-responsive Q-type C2H2 transcription factors in potato

Fig. 3

Expression of StZFP2-like genes in excised and leaves infested by Manduca sexta. Mean transcript level is, 2^− dCT with (dCT = CT of test gene-CT of exogenous control gene). Excised control leaves in (blue) and excised infested leaves in (red) are shown at each time point. Each value is the average of three biological replicates. Significant differences in control leaves over time are shown with capital letters. Significant differences in infested leaves over time are shown in lower case letters. Significant differences between control and infested leaves at the same time point is shown with an asterisk (*). Error bars represent standard deviation. Excised potato leaves from 19 day-old plantlets (cv, ‘Kennebec’) were infested with 3–4, 4th instar Manduca sexta larvae for 20 min. If any of the larva were not eating within 2–3 min, they were replaced with new larvae and at 20 min larvae were removed. Leaves were harvested (at 20, 40, 80, 160 and 1290 min) and immediately frozen in liquid nitrogen and stored at − 80 °C until RNA extraction. Leaf area consumed was calculated using Phenophyte [34], during a 20 min interval it ranged from 16 to 35%

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