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Fig. 2 | BMC Research Notes

Fig. 2

From: Tumor analysis: freeze–thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells

Fig. 2

Transcriptomic signatures of fresh and freeze/thawed CSCs. a Flow cytometry analysis of freshly dissociated tumor cells and spheroids derived from the tumor after 15 days of culture in low serum/low adherence conditions. The dot plot is representative of data obtained from all 15 tumors. b Heatmap showing RNA expression by spheroids derived from fresh or frozen tumor cells from three different patients. Hierarchical clustering was performed on Robust Multi-Array Average (rma)-normalized expression data using the One minus Pearson’s correlation method. c Overall, 274 genes showed differential expression in spheroids isolated from fresh and frozen tumors. Network representation of GO terms (biological process) significantly enriched in the 224 genes downregulated in freeze/thawed mammospheres as compared to fresh ones. Similar go terms were spatially clustered and colors represent corrected p-values

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