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Table 1 Purification of cellulase from crude sample of Paenibacillus sp.

From: Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses

Purification steps Volume (ml) Total protein (mg) Total activity (U) Specific activity (U/mg) Yield (%) Purification fold
Crude extract 1500 300 81,788 273 100 1
80% ammonium sulfate saturation 60 160 75,121 469 91.8 1.7
DEAE-cellulose column chromatography 55 40 64,525 1613 78.9 5.9
CM-cellulose column chromatography 25 17 29,210 1718 35.7 9.7