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Table 1 Purification of cellulase from crude sample of Paenibacillus sp.

From: Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses

Purification steps

Volume (ml)

Total protein (mg)

Total activity (U)

Specific activity (U/mg)

Yield (%)

Purification fold

Crude extract

1500

300

81,788

273

100

1

80% ammonium sulfate saturation

60

160

75,121

469

91.8

1.7

DEAE-cellulose column chromatography

55

40

64,525

1613

78.9

5.9

CM-cellulose column chromatography

25

17

29,210

1718

35.7

9.7