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Fig. 1 | BMC Research Notes

Fig. 1

From: ER stress regulates alkaline phosphatase gene expression in vascular smooth muscle cells via an ATF4-dependent mechanism

Fig. 1

ALP expression and activity are ATF4-dependent in VSMCs. VSMCs were treated with 0.4 μg/ml TM or 0.2 μg/ml TG for 24 h. a ALP mRNA levels measured by qPCR. b ALP activity. c, d Western blotting for Grp78 and Grp94 chaperones indicated that ER stress was activated. e QPCR analysis of ALP mRNA expression in VSMCs treated with IRE1, ATF6, PERK or ATF4 siRNA or non-targeting siRNA (Neg Ctrl) and then TM or TG for 24 h. f QPCR analysis of ATF4 mRNA expression in VSMCs treated with ATF4 siRNA or non-targeting siRNA. g ALP activity in VSMCs treated with ATF4 siRNA or non-targeting siRNA. * p < 0.05, ** p < 0.01, *** p < 0.001

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BMC Research Notes

ISSN: 1756-0500

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