Fig. 2

ALP promoter activity in VSMCs in response to ER stress and ATF4 knock-down. a Schematic showing ALP promoter constructs for luciferase assay. Construct 1 is the full length fragment, constructs 2–10 were derived by deleting increasing portions of the full length. Based on [14]. b Activity of luciferase expressed from ALP promoter constructs in VSMCs, n = 1. VSMCs were transfected with constructs for 24 h and then treated with 0.4 μg/ml TM or 0.2 μg/ml TG for 24 h. c–f Activity of luciferase expressed from ALP promoter constructs 3, 5, 6 and 7 in VSMCs treated with ATF4 siRNA or non-targeting siRNA, n = 3