Effects of lyophilization and storage temperature on Wuchereria bancrofti antigen sensitivity and stability

Table 1 Results of Alere FTS testing at baseline, one and 3 months storage at different temperatures

Samples	Microfilaria/ml of blood	Baseline testing	Results at 1 month			Results at 3 months
			4 °C	28 °C	40 °C	4 °C	28 °C	40 °C
A	1	+++	+++	+	+++	+++	+	++
B	1	+++	+++	++	++	+	−	+
C	6	+	+	+	+	+	+	+
D	43	++	+	+	+	+	+	−
E	7	+++	++	++	++	++	−	+
F	1	+++	++	++	+	++	+	+
G	6	+++	++	++	++	+	−	−
H	1	++	−	−	−	−	−	−
I	57	+++	+++	+++	++	++	+	+
J	2	++	+++	+++	+++	+	−	−
Number positive		10	9	9	9	9	5	6
Sensitivity		100%	90%	90%	90%	90%	50%	60%
Mean intensity score		2.5	2.0	1.7	1.7	1.4	0.5	0.7
Standard deviation		0.707	1.054	0.949	0.949	0.843	0.527	0.675
Standard error of mean		0.224	0.333	0.300	0.300	0.267	0.167	0.213
p values			0.188	0.055	0.055	0.008	0.004	0.004