Fig. 2
From: The jasmonate receptor COI1 is required for AtPep1-induced immune responses in Arabidopsis thaliana
Elicitor-induced MAPK activation in coi1-16 mutants. Phosphorylation of MPK6, MPK3, and MPK4/11 in 14-day-old seedlings treated with 200 nM flg22, 200 nM elf18, or 1 µM AtPep1 for 10 min compared to a mock control. Total protein was extracted from twelve seedlings and analyzed by immunoblot using anti-p42/p44 MAPK (Cell Signaling) and anti-rabbit-HRP (Sigma Aldrich) antibodies. The membrane was stained with Coomassie Brilliant Blue (CBB) as a measure of sample loading. Three experimental replicates were performed with similar results