Fig. 3
From: The jasmonate receptor COI1 is required for AtPep1-induced immune responses in Arabidopsis thaliana
Analysis of AtPep1-induced gene expression in coi1-16 mutants. Twelve 14-day-old Col gl1 and coi1-16 seedlings were treated with water (−) or 1 µM AtPep1 (+) for 120 min prior to RNA extraction. Quantitative real-time PCR was used to assess expression levels of the MAPK specific gene FRK1 (A), the MAPK dominant genes CYP81F2 (B) and WAK2 (C), the MAPK-CDPK synergistic genes NHL10 (D), CYP82C2 (E), and PER4 (F), the CDPK specific gene PHI-1 (G), the PAMP-induced genes FMO1 (H) and At1g51890 (i), and the AtPep precursor genes PROPEP1 (J), PROPEP2 (K), and PROPEP3 (L). Values are means + standard deviations (n = 3 technical replicates from the same cDNA), normalized against the relative average expression of UBOX from the same sample. A total of three independent experimental replicates were performed with similar results. Statistically significant groups (p < 0.05) are indicated with lower-case letters based on a one-way ANOVA followed by Tukey’s post-test