Table 1 Primer sequence for amplification of all coding regions of SLC26A4 gene for HRM mutations analysis
Exon | Amplicon | Primer sequence | Amplicon size (bp) |
|---|---|---|---|
1 | Ex 1 | 5′-GGGTGGCCCCTGCGTGG-′3 | 257 |
5′-CTCACCTGTCTCTGCTCGC-′3 | |||
2 | Ex 2-1 | 5′-CGTGTCCTCCCTCCTCGCT-′3 | 113 |
5′-GCGCCGCGACACCATGTAG-′3 | |||
Ex 2-2 | 5′-GCCGCAGCTCCCCGAGTA-′3 | 166 | |
5′-TTCTCTCTACGCAGGCCCGC-′3 | |||
3 | Ex 3 | 5′-GCTTTTTGACAGTTGT-′3 | 221 |
5′-CTATGGTAGCTGGGG-′3 | |||
4 | Ex 4-1 | 5′-TGTAATCACTTTGCATG-′3 | 99 |
5′-GTCAGGATAGGGAAAA-′3 | |||
Ex 4-2 | 5′-ATGGTCTCTACTCTGC-′3 | 93 | |
5′-GTAAAATATACTTATAATTACC-′3 | |||
5 | Ex 5 | 5′-CTGATTAATTGTTAGAGACTT-′3 | 218 |
5′-CCTGTATAATTCCAACCAG-′3 | |||
6 | Ex 6 | 5′-CGTAGTTGATATTTGGTGGC-′3 | 248 |
5′-GGCCCAGACTCAGAGAATG-′3 | |||
7 | Ex 7 | 5′-GTGCTCGTGTGCGTGTAGC-′3 | 212 |
5′-CTTACCACAATTACTTCTATAGGAA-′3 | |||
8 | Ex 8 | 5′-CATCTTTTGTTTTATTTCAG-′3 | 125 |
5′-CTAAGAGGAACACCACAC-′3 | |||
9 | Ex 9 | 5′-CTAGGTTTTTGCCTCCTGAA-′3 | 185 |
5′-TATAAAACCAGTTCAGCAAAAGG-′3 | |||
10 | Ex 10-1 | 5′-TTGGACCACCACGCAGAG-′3 | 208 |
5′-GACGGCCGTGCGGGAA-′3 | |||
Ex 10-2 | 5′-TTCTCTTGTTTTGTGGC-′3 | 161 | |
5′-TTGTCCTGCTAAGCTC-′3 | |||
11 | Ex 11 | 5′-CCTTTTCATAGGAGGTGTGTGTC-′3 | 133 |
5′-CGGTATGCAGAGAAGCAGG-′3 | |||
12 | Ex 12 | 5′-CACAGCCTTCTCTGTCT-′3 | 146 |
5′-AATATAGGTGGTAGGTGACT-′3 | |||
13 | Ex 13 | 5′-ATTTTTTTCCCTAGGT-′3 | 178 |
5′-AGGAAGCTCAGAGTGT-′3 | |||
14 | Ex 14 | 5′-TTCCAAAATACGGCTGTTC-′3 | 154 |
5′-AGTCCAGCAAATGTCTCACA-′3 | |||
15 | Ex 15 | 5′-TTGAAATTATTTAATCCCAGACAA-′3 | 179 |
5′-TCTCAAAAGAGGTTAGAAAACAAAT-′3 | |||
16 | Ex 16 | 5′-TTGACATTTATTTCCAA-′3 | 141 |
5′-GGGGGAAAAGAAAGATGTC-′3 | |||
17 | Ex 17-1 | 5′-GACAATTAAGTTGACAGTGTT-′3 | 182 |
5′-GGAACGTTCACTTTGACT-′3 | |||
17 | Ex 17-2 | 5′-GTGGATTGGAACTCTGAGC-′3 | 150 |
5′-GTATAATTCAGAAAACCAGAACC-′3 | |||
18 | Ex 18 | 5′-GAATTATGGGCAGATAAGG-′3 | 159 |
5′-GGCTTACGGGAAAGTCTT-′3 | |||
19 | Ex 19-1 | 5′-TGAGCAATGATGCCAC-′3 | 247 |
5′-AACCTTGACCCTCTTGAG-′3 | |||
Ex 19-2 | 5′-GGTTCTTTGACGACAACA-′3 | 150 | |
5′-AAAAGATACATCTGTAGAAAG-′3 | |||
20 | Ex 20 | 5′-TGCTATTCTATTTCTACC-′3 | 142 |
5′-TTCAGAAGAAAATGATCAT-′3 | |||
21 | Ex 21 | 5′-ATCAACACTTTGTTTTCC-′3 | 91 |
5′-TATTCCTTGCTCATAGAG-′3 |