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Fig. 1 | BMC Research Notes

Fig. 1

From: Exploring the differences between the three pyruvate kinase isozymes from Vibrio cholerae in a heterologous expression system

Fig. 1

VcIIIPK in BL21(DE3)pLysS strain. a Time course of growth curves of vcIpk, vcIIpk and vcIIIpk constructs before (non-induction) and after induction with 0.6 mM IPTG. The error bars represent the standard deviation of three independent experiments. b SDS-PAGE gels (12%) show the time course of VcIPK, VcIIPK or VcIIIPK before and after induction. The migration of a previously purified VcIPK used as marker is shown in Lane C and the low range molecular weights (Sigma Marker) are in lane M. c Agarose gel (1%) of the vcIpk, vcIIpk and vcIIIpk constructs before and after 15 h of induction. DNAs were digested with NdeI and BamH1. Two bands of ~ 1650 and ~ 650 bp corresponded to the PK genes and to a fragment of pLysS vector, respectively. d Agarose gel (1%) of the time course of vcIIIpk construct before and 3, 6, 9 and 15 h after induction. DNA was digested as in c and the same bands pattern was observed. In c and d 1 Kb Plus DNA ladder (Invitrogen) were used as markers (M). bd Performed from one of the experiments of a

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