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Fig. 2 | BMC Research Notes

Fig. 2

From: Exploring the differences between the three pyruvate kinase isozymes from Vibrio cholerae in a heterologous expression system

Fig. 2

VcIPK, VcIIPK and VcIIIPK in Origami B(DE3)pLysS (a, b) and in BL21-AI (c, d) strains. Time course of growth curves of vcIpk, vcIIpk and vcIIIpk constructs before (non-induction) and after induction with 0.6 mM IPTG at 37 °C (a) or 1.2 mM IPTG plus 0.25% L-arabinose at 25 °C (c). The error bars represent the standard deviation of three independent experiments. b and d SDS-PAGE (12%) show the time course of VcIPK, VcIIPK or VcIIIPK before (n-i) and after 3, 6, 9 or 15 h of induction. The migration of a previously purified VcIPK used as marker is shown in Lane C and the low range molecular weights (Sigma Marker) are in lane M

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