Table 2 PCR assays used in the study
From: Molecular investigations of viral meningitis among HIV-infected adults in Accra, Ghana
Virus | Primers oligosequences (5′–3′) | Target region | References |
|---|---|---|---|
Enterovirus | 5′-CCCTGAATGCGGCTAATCC-3′, reverse primer, 5′-ATTGTCACCATAAGCAGCCA-3′ and a probe, 5′-AACCGACTACTTTGGGTGTCCGTGTTTC-3′ | 5′ UTR | [22] |
Mumps | Forward primer (SH61F) 5′-GTGACCCTGCCGTTGCA-3′, reverse primer (SH147R), 5′-GTTATGATCAGAGAGAGAAGAATTAGCAATAG-3′ and probe (SH79P2) 5′-TATGCCGGCGATCCAACCTCCCTTATA-3′ | (SH) gene | [23] |
HSV-1 | Forward primer (HSV1UP), 5′-CGGCCGTGTGACACTATCG-3′, reverse primer (HSV1DP), 5′-CTCGTAAAATGGCCCCTCC-3′ and probe (HSV1P), 5′-CCATACCGACCACACCGACGAACC-3′ | (gD) gene | [24] |