Fig. 1
CaV1.3b current inhibition and kinetic changes produced by M1R stimulation are CaVβ-subunit dependent. a Representative current traces from CaV1.3b coexpressed with β1b, β2a, β3 or β4 before (black) or 1 min after applying 10 μM Oxo-M (red). b Current traces from a were normalized to the end of the test pulse. c Summary of Oxo-M inhibition of CaV1.3b with different CaVβ-subunits. Maximal inward current amplitudes were measured after the onset of the test pulse using a trough seeking function (peak current). Percent of current inhibition was calculated as: %Iinhib= 100*(ICTL–IDRUG)∕ICTL , where ICTL and IDRUG are the average maximum current amplitude of 5 traces prior to and after 1 min of application of test material (unless otherwise noted). d Schematic of quantification of kinetic changes. e, f Summary of kinetic changes (n = 4–6, ***P < 0.001, **P < 0.05) open bars, control; hatched bars, Oxo-M. e Time to peak (TTP) was measured using a minimum seeking function in Signal within the test pulse duration. f Current remaining (r40) was measured from an average of five normalized current traces per condition using the equation: r40 = 100*Iend∕Ipeak , where r40 is the percent of the maximum inward current remaining at the end of a 40 ms test pulse; Iend is the current amplitude at the end of the test pulse; Ipeak is the maximum inward current measured during the test pulse