Fig. 2
M1Rs but not D2Rs inhibit recombinant L-current. a Comparison of M1R and D2R signaling pathways that inhibit L-VGCC activity. b Time course of Oxo-M applied at time 0 for CaV1.3b-β2a current. c Time course of 10 nM quin applied at time 0 for CaV1.3b-β2a current. Inset: (left) Individual current traces before (black) and after 1 min of quin, scale bar = 0.5 nA. (right) Normalized traces. d Time course of Oxo-M applied at time 0 for CaV1.3b-β3 current. e Time course of 0.5 μM quin applied at time 0 for CaV1.3b-β3 current. Inset: same as c, scale bar = 1 nA. f Concentration–response curve of quin on CaV1.3b-β2a (filled circles) and CaV1.3b-β3 (open circles) currents (n = 2–5). g Summary of kinetic analysis