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Table 2 Conditions tested for isolation of splenic mononuclear cells

From: Limitations in the isolation and stimulation of splenic mononuclear cells in a dasyurid marsupial, Phascogale calura

Date Sex (number of individuals pooled) Age (months) Conditions tested Spleen cells
Speed (g) Time (min) RBCL WBC count Recovery (%) Viability (%)
18 April 2017 F (1) 9 400 25 No 3.5 × 107 0.5 86
2 May 2017 M (1) 10 400 20 Yes 8.9 × 107 0.2 97
22 May 2017 M (1) 10 400 25 Yes 1.7 × 107 0.6 76
M (1) 10 400 25 Yes 7.1 × 107 1.2 82
29 June 2017 F (1) 11 400 25 Yes 6.5 × 106 0.5 88
6 July 2017 M (2) 12 400 25 Yes 3.2 × 107 0.1 72
18 July 2017 F (3) 12 400 25 Yes 8.8 × 107 0.1 88
25 July 2017 F (2) 12 400 20 Yes 1.9 × 107 0.0 68
M (2) 12 400 20 Yes 5.0 × 107 0.1 88
1 Aug 2017 M (2) 13 350 20 Yes 1.4 × 107 0.3 88
M (2) 13 400 15 Yes 1.5 × 107 0.2 75
23 Aug 2017 F (2) 13 400 15 No 2.3 × 107 1.0 87
8 Sept 2017 F (2) 14 400 15 No 3.1 × 107 0.1 78
4 Oct 2017 F (1) 4 400 15 No 2.1 × 107 0.7 85