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Table 2 Conditions tested for isolation of splenic mononuclear cells

From: Limitations in the isolation and stimulation of splenic mononuclear cells in a dasyurid marsupial, Phascogale calura

Date

Sex (number of individuals pooled)

Age (months)

Conditions tested

Spleen cells

Speed (g)

Time (min)

RBCL

WBC count

Recovery (%)

Viability (%)

18 April 2017

F (1)

9

400

25

No

3.5 × 107

0.5

86

2 May 2017

M (1)

10

400

20

Yes

8.9 × 107

0.2

97

22 May 2017

M (1)

10

400

25

Yes

1.7 × 107

0.6

76

M (1)

10

400

25

Yes

7.1 × 107

1.2

82

29 June 2017

F (1)

11

400

25

Yes

6.5 × 106

0.5

88

6 July 2017

M (2)

12

400

25

Yes

3.2 × 107

0.1

72

18 July 2017

F (3)

12

400

25

Yes

8.8 × 107

0.1

88

25 July 2017

F (2)

12

400

20

Yes

1.9 × 107

0.0

68

M (2)

12

400

20

Yes

5.0 × 107

0.1

88

1 Aug 2017

M (2)

13

350

20

Yes

1.4 × 107

0.3

88

M (2)

13

400

15

Yes

1.5 × 107

0.2

75

23 Aug 2017

F (2)

13

400

15

No

2.3 × 107

1.0

87

8 Sept 2017

F (2)

14

400

15

No

3.1 × 107

0.1

78

4 Oct 2017

F (1)

4

400

15

No

2.1 × 107

0.7

85