Fig. 1
From: Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue
Schematic illustration of the experimental setup for Ussing chamber experiments. The composition of the serosal and mucosal buffer solutions with or without chloride is given in Additional file 1: Table S1. After an equilibration period with chloride-containing or chloride-free buffer solutions, RSV (Sigma-Aldrich, 300 µM) or ethanol (control, 20 µl/10 ml) were added to the mucosal compartment. After 30 min, glucose (10 mM mucosal) was applied in order to induce Na+-coupled glucose transport which is known to be inhibited by RSV [22, 25]. After another 10 min, carbachol (10 µM serosal, Sigma-Aldrich) was added to stimulate Ca2+-induced Cl− secretion (ΔIsc carbachol 1) [3]. The buffers were changed to chloride-containing buffers in all chambers and carbachol was added once again (ΔIsc carbachol 2). Mucosal additions exceeding 1 mM were osmotically balanced by the serosal addition of mannitol (non-absorbable, non metabolisable sugar)