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Fig. 1 | BMC Research Notes

Fig. 1

From: Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue

Fig. 1

Schematic illustration of the experimental setup for Ussing chamber experiments. The composition of the serosal and mucosal buffer solutions with or without chloride is given in Additional file 1: Table S1. After an equilibration period with chloride-containing or chloride-free buffer solutions, RSV (Sigma-Aldrich, 300 µM) or ethanol (control, 20 µl/10 ml) were added to the mucosal compartment. After 30 min, glucose (10 mM mucosal) was applied in order to induce Na+-coupled glucose transport which is known to be inhibited by RSV [22, 25]. After another 10 min, carbachol (10 µM serosal, Sigma-Aldrich) was added to stimulate Ca2+-induced Cl secretion (ΔIsc carbachol 1) [3]. The buffers were changed to chloride-containing buffers in all chambers and carbachol was added once again (ΔIsc carbachol 2). Mucosal additions exceeding 1 mM were osmotically balanced by the serosal addition of mannitol (non-absorbable, non metabolisable sugar)

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