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Fig. 1 | BMC Research Notes

Fig. 1

From: Increase of CaV3 channel activity induced by HVA β1b-subunit is not mediated by a physical interaction

Fig. 1

The β1b-subunit increases the current density in all three LVA channels. ac Representative whole-cell currents recorded at -30 mV from HEK-293 cells transfected with each of the CaV3 channels (CaV3.1, CaV3.2 or CaV3.3) alone or together with the β1b-subunit. Patch-clamp experiments were performed by using a HP of − 100 mV and 5 mM CaCl2 as charge carrier. df Current–voltage (IV) relationships for the same channels as in ac. For IV plots data were obtained from currents evocated from − 80 mV to + 80 mV in 10 mV steps; current amplitudes were normalized by cell capacitance to obtain current density values. Smooth lines are fits to data with a modified Boltzmann function (see Experimental Procedures). The corresponding parameters are shown in Additional file 1. gi Current density (mean ± SEM) at − 30 mV calculated for HEK-293 cells transfected with the indicated CaV3 channels alone or together with β1a, β1b, β2a, β3 or β4 subunits. Only β1b increases current density significantly when transfected with any of the CaV3 channels. Data were normalized to the current density values obtained when CaV3 channels were transfected alone. *Statistical significance when using ANOVA followed by Dunnett’s multiple comparison test (P < 0.05). The number of studied cells varied from 5 to 29

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