Fig. 3
From: Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147
Redox titration of roGFP1 and roGFP1-Se147. Constructs expressed in HEK293T. Following purification roGFP constructs were incubated with 10Â mM lipoate buffer with increasing ratios oxidized:reduced lipoate. a Normalized excitation spectra for roGFP1 in lipoate buffers. b Calculated 405/470 ratio of roGFP1 in lipoate buffers. c Normalized excitation spectra for roGFP1-Se147 in lipoate buffers. d Calculated 405/470 ratio of roGFP1-Se149 in lipoate buffers